Defending champion Jessica Pegula defeated Amanda Anisimova in the final, 6–3, 2–6, 6–1 to win the women's singles tennis title at the 2024 Canadian Open. It was her third WTA 1000 title and sixth career WTA Tour title. Pegula was the first player to defend the title since Martina Hingis in 2000. Ranked No. 132, Anisimova was the lowest-ranked finalist at the event since Alycia Moulton in 1984.