The Jacksonville State Gamecocks softball team represents Jacksonville State University in NCAA Division I college softball. As of July 1, 2023 the team participates in the Conference USA (C-USA). Prior to the move they were in the ASUN, a league in which it had been a member from 1995 to 2003. Prior to that JSU played in the OVC. The Gamecocks are currently led by head coach Julie Boland. Boland replaced Jana McGinnis in August of 2024, who retired after 31 years leading the Gamecocks. The team plays its home games at Jana McGinnis Field located on the university's campus. The Gamecocks reached the NCAA Super Regionals in 2009. The Gamecocks beat the University of Tennessee to advance.