The Florida Gators women's lacrosse team represents the University of Florida in the sport of college lacrosse. The Gators compete in Division I of the National Collegiate Athletic Association (NCAA) and are single-sport members of the Big 12 Conference, which they joined after the 2024 season. Previously, the team had competed in the American Athletic Conference, and before that the Big East Conference. Before joining Big East women's lacrosse, the Gators were members of the American Lacrosse Conference (ALC), which folded after the 2014 season due to aftereffects of conference realignment. They play their home games in Donald R. Dizney Stadium on the university's Gainesville, Florida campus, and are currently led by head coach Amanda O'Leary. The Gators have won regular-season conference titles in 11 of the 13 completed seasons of the women's lacrosse program's existence, with four each in the ALC and Big East plus three in The American. Additionally, they have won 10 conference tournament titles and advanced to the quarterfinals of the NCAA tournament six times, with their best NCAA finish being a semifinal berth in 2012.