The Bellarmine Knights are the teams representing Bellarmine University, located in Louisville, Kentucky, in intercollegiate sports as a member of the NCAA Division I ranks, primarily competing in the Atlantic Sun Conference (ASUN) for most sports, as of the 2020–21 academic year. The Knights previously competed in the Great Lakes Valley Conference (GLVC) of the NCAA Division II ranks from 1978–79 to 2019–20.